Journal: Oncotarget

Article Title: Nicotinic acetylcholine receptors induce c-Kit ligand/Stem Cell Factor and promote stemness in an ARRB1/ β-arrestin-1 dependent manner in NSCLC

doi:

Figure Lengend Snippet: Microarray was performed in ARRB1 depleted and nicotine stimulated A549 cells Nicotine induced and ARRB1 dependent genes from the microarray data were analyzed. We identified differentially regulated genes that were regulated by nicotine in a β-arrestin-1 dependent fashion and top 10 up/down regulated genes from the list were used for prognosis prediction. Assessment of the expression of these genes for smoking revealed that SCF (highlighted in red) strongly differentiated smokers from non-smokers implying an important role of this gene in lung carcinogenesis induced by smoking

Article Snippet: A human lung cancer tissue microarray slide (Catalog number IMH-358; Imgenex, San Diego, CA) was immunostained for SCF.

Techniques: Microarray, Expressing, Control

Journal: Clinical Cancer Research

Article Title: Reciprocal Modifications of CLIC4 in Tumor Epithelium and Stroma Mark Malignant Progression of Multiple Human Cancers

doi: 10.1158/1078-0432.ccr-06-1562

Figure Lengend Snippet: Fig. 1. Expression of CLIC4 is altered in several human cancer types. A, tumor array representing cDNAs derived from the ‘‘matched’’normal (N) and tumor (T) tissues (paired horizontally) of multiple human cancer types is probed with radioactively labeled CLIC4 or ubiquitin (inset) DNA probes. B, oncomine database (University of Michigan Comprehensive Cancer Center) was searched with‘‘CLIC4’’ as a query.Two examples of statistically significant changes (prostate/Lapointe/ Stanford University/P = 1.8 1021and lung/Bhattacharjee/Harvard Medical School/P = 3 109). Blue columns, normal tissues; red columns, cancerous tissues. Brackets,1.5 times interquartile range; dots, outliers; line within box, median; box boundaries, upper and lower quartiles.Two independent studies. C, tumor lysate dot blot representing 200 tumor samples (600 ng per spot) is probed with NH2-terminal CLIC4 antibody. ‘‘Matched’’normal (solid gray arrowhead) and tumor (solid black arrowhead) tissue samples are spotted vertically as a pair. Open circles are marker spots for an orientation.The experiment was repeated twice, and independent experiments with 10-fold less amount of lysate loaded per spot showed similar results.

Article Snippet: Immunohistochemistry, immunofluorescence, and tissue microarray staining Human tissue microarrays were obtained from multiple sources: TARP tissue arrays (National Cancer Institute), ‘‘matched’’ human tumor tissue arrays (Imgenex, San Diego, CA), Food and Drug Administration–standard normal and tumor tissue arrays (Biochain, Hayward, CA) and human cancer screen tissue arrays (Clinomics), prostate tumor arrays (Baylor Specialized Programs of Research Excellence), and human tissue arrays (Accumax) were used for CLIC4 expression and localization studies.

Techniques: Expressing, Derivative Assay, Labeling, Ubiquitin Proteomics, Dot Blot, Marker

Journal: Clinical Cancer Research

Article Title: Reciprocal Modifications of CLIC4 in Tumor Epithelium and Stroma Mark Malignant Progression of Multiple Human Cancers

doi: 10.1158/1078-0432.ccr-06-1562

Figure Lengend Snippet: Fig. 2. CLIC4 expression and subcellular localization are altered in human tumors. Human normal and matched tumor (roman numerals, tumor stage) tissue sections representing (A) esophagus, (B) kidney, and (C) colon from the tissue microarrays are immunostained with anti-CLIC4 antibody and visualized by bright-field microscopy under 40 magnification. Inset, lower magnification (10). Black arrows, CLIC4 localized to the nucleus in the normal tissues. Similar staining patterns of CLIC4 were found in multiple human tumor types. Representative immunohistochemical stainings.

Article Snippet: Immunohistochemistry, immunofluorescence, and tissue microarray staining Human tissue microarrays were obtained from multiple sources: TARP tissue arrays (National Cancer Institute), ‘‘matched’’ human tumor tissue arrays (Imgenex, San Diego, CA), Food and Drug Administration–standard normal and tumor tissue arrays (Biochain, Hayward, CA) and human cancer screen tissue arrays (Clinomics), prostate tumor arrays (Baylor Specialized Programs of Research Excellence), and human tissue arrays (Accumax) were used for CLIC4 expression and localization studies.

Techniques: Expressing, Microscopy, Staining, Immunohistochemical staining

Journal: Oncotarget

Article Title: KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells of multiple hepatocellular carcinoma subtypes

doi:

Figure Lengend Snippet: ( A and B ) Flow cytometric analysis of surface KIAA1114 expression in human CC cell lines (A) and AFP + and AFP − HCC cell lines (B) . (C) Immunoblot analysis of tissue lysates from normal liver (N), patient tumor (PT), and its adjacent normal tissue from the same donor (PN). β-actin served as an internal control.

Article Snippet: Human liver whole tissue lysate and paired primary tumor and normal tissues were purchased from Novus Biologicals.

Techniques: Expressing, Western Blot, Control

Journal: iScience

Article Title: Inhibition of the pseudokinase MLKL alters extracellular vesicle release and reduces tumor growth in glioblastoma

doi: 10.1016/j.isci.2022.105118

Figure Lengend Snippet:

Article Snippet: Human Brain Whole Tissue Lysate (Adult Whole Tumor) , Novus Biologicals , Cat# NB820-59423.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, EdU Assay, Flow Cytometry, Imaging, Cell Counting, Luciferase, Viability Assay, Isolation